The success of an autologous fat graft depends in part on its total stromal vascular fraction (SVF) and adipose-derived stem cells (ASCs). This is important when it comes to harvesting of fat for fat transfer such as in Brazilian Butt Lift.
Subcutaneous fat tissues from the abdomen, flank, or axilla were harvested in the study conducted by Iyyanki from patients of various ages by mechanical liposuction, direct surgical excision, or Coleman's technique with or without centrifugation.
Compared with Coleman's technique without centrifugation, direct excision yielded significantly more ASCs (P < .001) and total SVF cells (P = .007); liposuction yielded significantly fewer ASCs (P < .001) and total SVF cells (P < .05);
Coleman's technique with centrifugation yielded significantly more total SVF cells (P < .005), but not ASCs.
The total number of SVF cells in fat harvested from the abdomen was significantly larger than the number in fat harvested from the flank or axilla (P < .05).
Adipose tissue harvested from the abdomen through direct excision or Coleman's technique with centrifugation was found to yield the most SVF cells and ASCs.
Iyyanki, et al. Harvesting technique affects adipose-derived stem cell yield. Aesthetic Surgery J. 2015 May;35(4):467-76. doi: 10.1093/asj/sju055. Epub 2015 Mar 18.